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KMID : 1020220120120040733
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Journal of Korean Society of Dental Hygiene
2012 Volume.12 No. 4 p.733 ~ p.739
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Comparative study on survival rate of human gingival fibroblasts stored in different storage media
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Lee Hee-Su
Lim You-Sun
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Abstract
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Objectives: To Compare the degree of survival rate of gingival fibroblasts, which is concerned with teeth adherence based on the type of avulsed tooth’s storage solution.
Methods: Different media gingival fibroblasts were stored in Dulbecco’s modified Eagle’s medium(DMEM), Hank’s balanced salt solution(HBSS), milk, saline, and green tea in for 1, 2, 3 hours. And, MTT assay was conducted to compare survival rate of human gingival fibroblasts.
Results: 1. The survival rate of gingival fibroblasts in DMEM and HBSS was higher than thoes in other storage media( Milk> Saline> Green tea). 2. The survival rate of gingival fibroblasts in milk, saline and green tea decreased as time passed. 3. Because of low osmotic pressure, green tea showed decrease of survival rate of gingival fibroblasts.
Conclusions: DMEM and HBSS were the most effective storage media for gingival f ibroblast. Among milk, saline, green tea, milk is most effective storage media for keeping gingival fibroblasts. Milk is recommended for storage media of avulsed tooth for keeping viability of cells.
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KEYWORD
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Avulsed Teeth, Gingival Fibroblast, Storage Solution
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